Background. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are providing new possibilities for the\nbiological study, cell therapies, and drug discovery. However, the ion channel expression and functions as well as regulations in\nhiPSC-CMs still need to be fully characterized. Methods. Cardiomyocytes were derived from hiPS cells that were generated from\ntwo healthy donors. qPCR and patch clamp techniques were used for the study. Results. In addition to the reported ion\nchannels, INa, ICa-L, ICa-T, If, INCX, IK1, Ito, IKr, IKs IKATP, IK-pH, ISK1ââ?¬â??3, and ISK4, we detected both the expression and currents of\nACh-activated (KACh) and Na+-activated (KNa) K+, volume-regulated and calcium-activated (Cl-Ca) ClâË?â??, and TRPV channels.\nAll the detected ion currents except IK1, IKACh, ISK, IKNa, and TRPV1 currents contribute to AP duration. Isoprenaline increased\nICa-L, If, and IKs but reduced INa and INCX, without an effect on Ito, IK1, ISK1ââ?¬â??3, IKATP, IKr, ISK4, IKNa, ICl-Ca, and ITRPV1. Carbachol\nalone showed no effect on the tested ion channel currents. Conclusion. Our data demonstrate that most ion channels, which are\npresent in healthy or diseased cardiomyocytes, exist in hiPSC-CMs. Some of them contribute to action potential performance\nand are regulated by adrenergic stimulation.
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